Spheroids, which are near-spherical multicellular aggregates, are one of the most common types of three-dimensional (3D) cell cultures. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. However, methodological transparency and guidelines for spheroid research have not yet been established. Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. ![]() Robbins, 25 Elina Siljamäki, 21 Patrick Steigemann, 26 Gwen Sys, 27 Shuichi Takayama, 4 Anna Tesei, 24 Joeri Tulkens, 1, 2 Michiel Van Waeyenberge, 3 Jo Vandesompele, 5, 6 Glenn Wagemans, 1, 2 Claudia Weindorfer, 19 Nurten Yigit, 5, 6 Nina Zablowsky, 26 Michele Zanoni, 24 Phillip Blondeel, 3 and Olivier De Wever 1, 2 Kunz-Schughart, 22 Sebastiaan Maes, 3 Christophe Mangodt, 1, 2 Pieter Mestdagh, 5, 6 Soňa Michlíková, 22 Maria José Oliveira, 12 Francesco Pampaloni, 23 Filippo Piccinini, 24 Cláudio Pinheiro, 1, 2 Jennifer Rahn, 25 Stephen M. Dmitriev, 18 Helmut Dolznig, 19 Suzanne Fischer, 1, 2 Christian Gespach, 20 Vera Goossens, 7 Jyrki Heino, 21 An Hendrix, 1, 2 Peter Horvath, 17 Leoni A. Arne Peirsman, # 1, 2, 3 Eva Blondeel, # 1, 2 Tasdiq Ahmed, 4 Jasper Anckaert, 5, 6 Dominique Audenaert, 7 Tom Boterberg, 8 Krisztina Buzas, 9 Neil Carragher, 10 Gastone Castellani, 11 Flávia Castro, 12 Virginie Dangles-Marie, 13, 14 John Dawson, 10 Pascal De Tullio, 15 Elly De Vlieghere, 1, 2 Sándor Dedeyne, 1, 2 Herman Depypere, 16 Akos Diosdi, 17 Ruslan I.
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